Objective,2,To explore the lumen sterilization challenge device in three types of hydrogen peroxide low-temperature plasma sterilization effect further improve the low-temperature plasma sterilization device qualified rate.,Methods,2,In November 2021-May 2022, CSSD in our hydrogen peroxide plasma five card sterilization challenge device respectively in three types of low temperature plasma sterilization device exhaust vent positions, for different internal diameters, different lengths, different materials of medical metal and non-metal cavity instruments in three types of different cycle program instrument sterilization effect.,Results,2,The hydrogen peroxide plasma class 5 card sterilization challenge device is placed at the exhaust port of STERRAD NX sterilization device, with standard cycle and high sterilization qualification rate (,P,>,0.05) with internal diameter of 1mm; only compatible with the advanced circulation of the machine, with low sterilization qualification rate. Hydrogen peroxide plasma type 5 card sterilization challenge device is placed at the STERRAD 100 S sterilizer vent, Short cycles and long cycles for common metallic nonmetallic medical devices, High sterilization qualified rate, No sterilization effect (,P,>,0.05); For the inner diameter of 0.7 mm, Low short cycle sterilization rate of 500 mm, High qualified rate of long-cycle sterilization, The sterilization effect was statistically different (,P,<,0.01); The inner diameter is 1mm, A length of 850 mm single channel or no lumen soft mirror is only compatible with the advanced cycle of the machine, High qualified rate of sterilization. Hydrogen peroxide plasma type 5 card sterilization challenge device is placed at the STERRAD 100NX sterilization device outlet, the standard cycle for common metal non-metallic medical devices and internal diameter of 0.7 mm, length of 500 mm stainless steel medical lumen sterilization qualified rate; internal diameter of 1mm, length of 850 mm single channel or no cavity soft mirror can only be compatible with the soft mirror cycle of the machine, the sterilization qualified rate is also high.,Conclusion,2,STERRAD NX standard cycle sterilized common metal and non-metallic medical devices with high qualified rate; STERRAD 100S short cycle sterilized common metal and non-metallic medical devices with 0.7 mm and 500 mm Stainless steel medical lumen and internal diameter 1mm, length 850 mm, STERRAD 100NX standard cycle for common metal non-metallic medical instruments and internal diameter 0.7 mm, length 500 mm, and 500 mm, soft mirror cycle for internal diameter 1mm, length 850 mm single channel or without sterilization, sterilization qualified rate.