Study on a novel monitoring method of residual protein in cleaning effect of reusable medical devices

Junjun Shi; Yusheng Li; Yiqing Chen; Wei Wang

doi:10.11910/j.issn.2791-2043.2023.1.05

Your Location：

Home >

Browse articles >

Study on a novel monitoring method of residual protein in cleaning effect of reusable medical devices

MONOGRAPHIC STUDY | Updated：2023-03-27

- Study on a novel monitoring method of residual protein in cleaning effect of reusable medical devices
- Sterile Supply Vol. 2, Issue 1, (2023)
- Affiliations：
  
  1.Nanjing Jusha Medical Technology Co., Ltd., 210000, Nanjing, Jiangsu, China
- Author bio：
- Funds：
- DOI：10.11910/j.issn.2791-2043.2023.1.05
  CLC：
Scan for full text
Shi Junjun,Li Yusheng,Chen Yiqing,et al.Study on a novel monitoring method of residual protein in cleaning effect of reusable medical devices[J].Sterile Supply,2023,02(01):34-40.
DOI：

Shi Junjun,Li Yusheng,Chen Yiqing,et al.Study on a novel monitoring method of residual protein in cleaning effect of reusable medical devices[J].Sterile Supply,2023,02(01):34-40. DOI： 10.11910/j.issn.2791-2043.2023.1.05.

摘要

Abstract

Objective,2,To study the application of a new detection method of residual protein.,Methods,2,The detection range and anti-interference detection ability of the new residual protein monitoring method were tested and the data was compared with the UV-Vis OPA method. The influence of the differences among five different types of protein on the two detection results and the detection rate of residual protein in different devices were tested.,Results,2,The new residual protein detection method's detection limit was 0.1μg/mL, and it was insensitive to non-oxidizing interferents. The difference in protein is low, and the Cv value is 13.74. The detection rate of protein was better than that of UV-Vis OPA method, and the difference was significant (P > 0.01).,Conclusion,2,Compared with UV-vis OPA method, the new detection method of residual protein has higher sensitivity, a more comprehensive detection range, and a lower difference between proteins. It is effective to monitor the residual protein in device cleaning, and it is worth popularizing.

关键词

Keywords

Reusable medical devicesResidual protein testNew typeMonitor

references

Alfa M J . Medical instrument reprocessing: current issues with cleaning and cleaning monitoring[J]. American journal of infection control, 2019, 47S:A10-A16.

HTM 01-01 2016. Health Technical Memorandum (HTM) 01-01 on the management and decontamination of surgical instruments (medical devices) used in acute care. National Health Service, 2016.

AAMI ST79. Comprehensive Guide to Steam Sterilization and Sterility Assurance in Health Care Facilities[S]. AAMI.

SHA R, ZHANG R M, HU Y Y, et al. Application of vacuum boiling washer combined with quantitative detection of protein residue in the cleaning of intervertebral foramen endoscopic instruments[J]. China Med Equip, 2021, 18(9): 52-55.

ISO/DIS 15883-5 Washer-disinfectors — Part 5: Performance requirements and test method criteria for demonstrating cleaning efficacy.

Reichelt W N , Waldschitz D , Herwig C , et al. Bioprocess monitoring: minimizing sample matrix effects for total protein quantification with bicinchoninic acid assay[J]. Journal of Industrial Microbiology & Biotechnology, 2016, 43(9):1271-1280.

Smith A, Letters S , Lange A , et al. Residual protein levels on reprocessed dental instruments[J]. Journal of Hospital Infection, 2005, 61(3):237-241.

Michels W , Roth K , Eibl R . Assessment of cleaning efficacy based on the protein-surface relationship. 2013(3).212-215

Diab-Elschahawi M, Balcky A, et al. Challenging the Sterrad 100NX Sterilizer with Different Carrier Materials and Wrapping under experimental “clean” and “dirty” conditions[J]. Am J Infect Control, 2010,38(10):806-810.

Pratical Guidelines for Infection Control in Health Care Facilities[S]. World Health Organization, 2004.

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

No data

Related Author

No data

Related Institution

No data

⁰