Quality Detection method on cleaning quality of silicone tube in phacoemulsification

Lijun Cai; Tingting Lin; Chufen Zhuang; Yu Zhang; Xingling Deng

doi:10.11910/j.issn.2791-2043.2024.2.05

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Quality Detection method on cleaning quality of silicone tube in phacoemulsification

MONOGRAPHIC STUDY | Updated：2026-01-13

- Quality Detection method on cleaning quality of silicone tube in phacoemulsification
- Sterile Supply Vol. 3, Issue 2, (2024)
- Affiliations：
  
  Central Sterile Supply Department, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, 510060, China
- Author bio：
  
  Xingling Deng at dxl129@163.com
- Funds：
- DOI：10.11910/j.issn.2791-2043.2024.2.05
  CLC：
- Published：15 August 2024
- Accepted：
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Cai Lijun,Lin Tingting,Zhuang Chufen,et al.Quality Detection method on cleaning quality of silicone tube in phacoemulsification[J].Sterile Supply,2024,03(02):81-85.
DOI：

Cai Lijun,Lin Tingting,Zhuang Chufen,et al.Quality Detection method on cleaning quality of silicone tube in phacoemulsification[J].Sterile Supply,2024,03(02):81-85. DOI： 10.11910/j.issn.2791-2043.2024.2.05.

摘要

Abstract

Objective

The present study intends to observe the cleaning effect of different detection methods in cleaning silicone tubes used in phacoemulsification.

Methods

A total of 100 silicone tubes were selected randomly after surgery. The silicone tubes were retained for ≤2 hours after surgery

and then washed with a high-pressure water gun at a flow rate of 12~14 ml/s. Adenosine Triphosphate (ATP) detection and quantitation of residual protein were performed on the samples before cleaning and after washing for 30 s

40 s

and 50 s

respectively

including the sample surface and the water after cleaning.

Results

According to the results before and after cleaning the silicone tube

there are significant differences in three methods of quantitation of residual protein

ATP detection in water sample

and ATP detection in sample surface (c

=8.6

0.05)

while having no difference between the three methods after washing for 30 s

40 s and 50 s

respectively (c

=4.918 and 5.571

0.05). A comparison of the means of ATP detection in water samples showed significant differences between rinses 30 s/40 s and 30 s/50 s. (Z=-7.45 and -0.08

0.05); pairwise contrast of ATP detection in sample surface for rinsing 30 s/40 s

40 s/50 s

and 30 s/50 s showed significant differences (Z=3.64

14.92

and 25.86

0.05). The quantitation of residual protein in silicone tubes showed pass rates of 84%

100%

and 100% for 30 s

40 s

and 50 s

respectively.

Conclusion

Quantitation of residual protein

ATP detection in water sample

and ATP detection in sample surface are available for monitoring the cleaning quality of silicone tube. The tube should be cleaned at a 12~14 ml/s flow rate and a washing time of ≥50 s.

关键词

Keywords

references

J. Wang , R.X. Gao , L.B. Zhang , et al , “ Comparative study on the cleaning effect of two kinds of ATP fluorescence detection reagents on medical devices ,” Chinese Journal of Disinfection , vol. 37 , pp. 724 - 726 , 2020 .

R. J. Olson , R. Braga-Mele, S. H. Chen et al., “Cataract in the adult eye pre ferred practice pattern®,” Ophthalmology, vol. 124 , no. 2 , pp. P1-P119, 2017.

N. Mamalis and D. F. Chang , “ Guidelines for the cleaning and sterilization of intraocular surgical instruments ,” Journal of Cataract and Refractive Surgery , vol. 44 , no. 6 , pp. 675 - 676 , 2018 .

E. Hernandez-Bogantes , A. Navas , A. Naranjo et al. , “ Toxic anterior segment syndrome: a review ,” Survey of Ophthalmology , vol. 64 , no. 4 , pp. 463 - 476 , 2019 .

T. Sanna , L. Dallolio , A. Raggi et al. , “ ATP bioluminescence assay for evaluating cleaning practices in operating theatres: applicability and limitations ,” BMC Infectious Diseases , vol. 18 , no. 1 , p. 583 , Nov 2018.

R. Sha , R.M. Zhang , Y.Y. Hu , et al , “ Application of vacuum boiling washer combined with quantitative detection of protein residue in the cleaning of intervertebral foramen endoscopic instruments ,” China Medical Equipment , vol. 18 , pp. 52 - 55 , 2021 .

X.J. He and G.Q. Jiang , “ Observation on the ATP bioluminescence assay used for evaluation of cleaning quality of medical instruments ,” Chinese Journal of Disinfection , vol. 34 , pp. 428 - 430 , 2017 .

M. D. Masia , M. Dettori , G. M. Deriu et al. , “ ATP bioluminescence for assessing the efficacy of the manual cleaning procedure during the reprocessing of reusable surgical instruments ,” Healthcare , vol. 9 , no. 3 , p. 352 , Mar 2021.

Y.W. Jiang and G.Y. Mo , “ Cleaning effect of biofilm cleaning agent combined with ultrasonic cleaning on lumen instruments ,” Nursing Practice and Research , vol. 13 , pp. 139 - 140 , 2016 .

S.M. Wei , “ Research progress on removal of biofilm attached to reusable surgical instruments in hospital disinfection supply center ,” Chinese General Practice Nursing , vol. 17 , pp. 2219 - 2222 , 2019 .

X.P. Luo , “ Function of medical high pressure water jet grout on preconditioning of uterine cavity aspiration tube ,” Chinese Journal of Practical Nursing , vol. 31 , pp. 663 - 665 , 2015 .

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